# BYOM, byom_doseresp_surv.m

**Table of contents**

## Contents

## About

- Author: Tjalling Jager
- Date: May 2019
- Web support: http://www.debtox.info/byom.html
- Back to index walkthrough_doseresp.html

BYOM is a General framework for simulating model systems in terms of ordinary differential equations (ODEs) or explicit functions. This package only supports explicit functions, which are calculated by simplefun.m, which is called by call_deri.m. The files in the BYOM engine directory are needed for fitting and plotting. Results are shown on screen but also saved to a log file (results.out).

**The model:** Log-logistic dose response fitting.

**This script:** Example for survival data, using the binomial likelihood function as the error model.

% Copyright (c) 2012-2019, Tjalling Jager, all rights reserved. % This source code is licensed under the MIT-style license found in the % LICENSE.txt file in the root directory of BYOM.

## Initial things

Make sure that this script is in a directory somewhere **below** the BYOM folder.

clear, clear global % clear the workspace and globals global DATA W X0mat % make the data set and initial states global variables global glo % allow for global parameters in structure glo global pri zvd % global structures for optional priors and zero-variate data diary off % turn of the diary function (if it is accidentaly on) % set(0,'DefaultFigureWindowStyle','docked'); % collect all figure into one window with tab controls set(0,'DefaultFigureWindowStyle','normal'); % separate figure windows pathdefine % set path to the BYOM/engine directory glo.basenm = mfilename; % remember the filename for THIS file for the plots glo.saveplt = 0; % save all plots as (1) Matlab figures, (2) JPEG file or (3) PDF (see all_options.txt)

## The data set

Data are entered in matrix form, time in rows, scenarios (exposure concentrations) in columns. First column are the exposure times, first row are the concentrations or scenario numbers. The number in the top left of the matrix indicates how to calculate the likelihood:

- -2 for binomial likelihood (dose-response fitting of survival data)
- -1 for multinomial likelihood (for survival data)
- 0 for log-transform the data, then normal likelihood
- 0.5 for square-root transform the data, then normal likelihood
- 1 for no transformation of the data, then normal likelihood

% Dieldrin survival data for guppies, at t=0 and the end of the test (7 % days). Exposure concentration is in ug/L. More replicates can be entered, % but only enter one exposure time! DATA{1} = [-2 7 0 20 3.2 18 5.6 18 10 8 18 2 32 0 56 0 100 0 ]; % Weight factors is now the initial number of animals at t=0 in each % replicate in the data set. Here, all treatments start with the same % number of animals, so we can define the weights factor with a shortcut. W{1} = 20*ones(size(DATA{1})-1); % 20 individuals in each treatment % Derive initial values from the data set, and plotting range for model % curve. The code below calls a small function with rules-of-thumb to come % up with relevant starting values. [Y0,ECx,logc] = startvals(DATA{1},W{1});

## Initial values for the state variables

Initial states, scenarios in columns, states in rows. First row are the 'names' of all scenarios.

X0mat(1,:) = DATA{1}(1,2); % assume second element of first row is the scenario (exposure duration) X0mat(2,:) = 0; % initial values state 1 (not used)

## Initial values for the model parameters

Model parameters are part of a 'structure' for easy reference.

glo.x_EC = 50; % the x in the ECx glo.logsc = 1; % plot the dose-response curve on log-scale % syntax: par.name = [startvalue fit(0/1) minval maxval]; par.ECx = [ECx 1 0 1e6]; % ECx, with x in glo.x_EC par.Y0 = [Y0 1 0 1]; % control response (survival probability) par.beta = [4 1 0 100]; % slope factor of the log-logistic curve

## Time vector and labels for plots

Specify what to plot. If time vector glo.t is not specified, a default is used, based on the data set. Note that t is now used for the exposure concentrations!

if glo.logsc == 1 % when plotting on log-scale ... % glo.logzero = 0.01; % option to override automatic position for plotting the control treatment on log-scale if isfield(glo,'logzero') && log10(glo.logzero) < logc(1) % if we modify the position of the control ... logc(1) = log10(glo.logzero) - log10(1.2); % also adapt the lower part of the plotting range end glo.t = [0,logspace(logc(1),logc(2),300)]; % use a log-spacing for the model curve % Note: t=0 needs to be in there for the calculation of the model for the control treatment else % otherwise ... glo.t = linspace(0,10^(logc(2)),300); % otherwise, use a fine linear spacing for the model curve end % specify the y-axis labels for each state variable glo.ylab{1} = 'survival probability'; % specify the x-axis label (same for all states) glo.xlab = ['concentration (',char(181),'g/L)']; glo.leglab1 = 'time '; % legend label before the 'scenario' number glo.leglab2 = '(d)'; % legend label after the 'scenario' number prelim_checks % script to perform some preliminary checks and set things up % Note: prelim_checks also fills all the options (opt_...) with defauls, so % modify options after this call, if needed.

## Calculations and plotting

Here, the functions are called that will do the calculation and the plotting. Profile likelihoods are used to make robust confidence intervals. Note that the dose-response curve is plotted on a log scale for the exposure concentration. If the control is truly zero, it is plotted as an open symbol, at a low concentration on the x-axis. However, for the fitting, it is truly zero.

opt_optim.fit = 1; % fit the parameters (1), or don't (0) opt_optim.it = 0; % show iterations of the optimisation (1, default) or not (0) opt_plot.annot = 1; % extra subplot in multiplot for fits: 1) box with parameter estimates, 2) overall legend % optimise and plot (fitted parameters in par_out) par_out = calc_optim(par,opt_optim); % start the optimisation calc_and_plot(par_out,opt_plot); % calculate model lines and plot them disp(['x in ECx is: ',num2str(glo.x_EC)])

Goodness-of-fit measures for each data set (R-square) 0.9945 Warning: R-square is not appropriate for survival data and needs to be interpreted more qualitatively! ================================================================================= Results of the parameter estimation with BYOM version 4.3 ================================================================================= Filename : byom_doseresp_surv Analysis date : 22-May-2019 (14:13) Data entered : data state 1: 8x1, survival data (dose-response). Search method: Nelder-Mead simplex direct search, 2 rounds. The optimisation routine has converged to a solution Total 107 simplex iterations used to optimise. Minus log-likelihood has reached the value 34.604115 (AIC=75.2082). ================================================================================= ECx 9.39 (fit: 1, initial: 7.483) Y0 0.9735 (fit: 1, initial: 1) beta 3.734 (fit: 1, initial: 4) ================================================================================= Time required: 0.1 secs Plots result from the optimised parameter values. x in ECx is: 50

## Profiling the likelihood

By profiling you make robust confidence intervals for one or more of your parameters. Use the name of the parameter as it occurs in your parameter structure *par* above. You do not need to run the entire script before you can make a profile.

Options for the profiling can be set using opt_prof (see prelim_checks or byom_bioconc_extra in the BYOM examples directory).

Note: in this example, the CI for the control response (*Y0*) runs into its upper boundary of 1 (which is no problem, as it implies 100% survival in the control).

Xing = calc_proflik(par_out,'ECx',opt_prof); % calculate a profile % Note that the confidence intervals our now returned in _Xing_ (as % crossings of the chi-square criterion), which can e.g., be used in later % plots (below, it is added to the annotation box for subsequent plotting). glo.str_extra{1} = sprintf('EC%1.0f: %6.4g (%4.4g - %4.4g)',glo.x_EC, par_out.ECx(1),Xing([1 end],1)); calc_and_plot(par_out,opt_plot); % calculate model lines and plot them

95% confidence interval from the profile ================================================================================= ECx interval: 7.379 - 11.56 Time required: 1.3 secs Plots result from the optimised parameter values.

## Likelihood region

Another way to make intervals on model predictions is to use a sample of parameter sets taken from the joint likelihood-based conf. region. This is done by the function calc_likregion.m. It first does profiling of all fitted parameters to find the edges of the region. Then, Latin-Hypercube sampling, keeping only those parameter combinations that are not rejected at the 95% level in a lik.-rat. test. The inner rim will be used for CIs on forward predictions.

Options for the likelihood region can be set using opt_likreg (see prelim_checks.m).

% UNCOMMENT LINE(S) TO CALCULATE opt_likreg.detail = 2; % detailed (1) or a coarse (2) calculation opt_likreg.subopt = 0; % number of sub-optimisations to perform to increase robustness opt_likreg.burst = 1000; % number of random samples from parameter space taken every iteration calc_likregion(par_out,500,opt_likreg); % second argument number of samples (-1 to re-use saved sample from previous runs)

Calculating profiles and sample from confidence region ... please be patient. Confidence interval from the profile (single parameter, 95% confidence) ================================================================================= ECx interval: 7.379 - 11.56 Warning: taking the highest parameter value as upper confidence limit Y0 interval: 0.879 - 1 beta interval: 2.255 - 6.663 Edges of the joint 95% confidence region (using df=p) ================================================================================= ECx interval: 6.738 - 12.64 Y0 interval: 0.8292 - 1 beta interval: 1.911 - 8.515 Starting with obtaining a sample from the joint confidence region. Bursts of 1000 samples, until at least 500 samples are accepted in inner rim. Currently accepted (inner rim): 0, 105, 208, 308, 410, Size of total sample (profile points have been added): 5050 of which accepted in the conf. region: 1609, and in inner rim: 545 Time required: 5.5 secs

## Plot results with confidence intervals

The following code can be used to make a standard plot (the same as for the fits), but with confidence intervals (and sampling error for survival data, if needed). Options for confidence bounds on model curves can be set using opt_conf (see prelim_checks).

Use opt_conf.type to tell calc_conf which sample to use: 1) Bayesian MCMC sample (default); CI on predictions are 95% ranges on the model curves from the sample 2) parameter sets from a joint likelihood region (limited sets can be used), which will yield (asymptotically) 95% CIs on predictions

% UNCOMMENT LINE(S) TO CALCULATE opt_conf.type = 2; % use the values from the slice sampler (1) or likelihood region (2) to make intervals out_conf = calc_conf(par_out,opt_conf); % calculate confidence intervals on model curves calc_and_plot(par_out,opt_plot,out_conf); % call the plotting routine again to plot fits with CIs

Amount of 0.4 added to the chi-square criterion for inner rim Full sample of 611 sets used. Calculating CIs on model predictions, using sample from joint likelihood region ... please be patient. Percentage of samples finished: 0 10 20 30 40 50 60 70 80 90 Time required: 6.2 secs Plots result from the optimised parameter values.